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Original Research |
Rajesh Nayak, PhD, National Center for Toxicological Research, United States Food and Drug Administration, Jefferson, Arkansas
Veronica Call, BS, Office of Regulatory Affairs/Arkansas Regional Laboratory, United States Food and Drug Administration, Jefferson, Arkansas
Pravin Kaldhone, MS, National Farm Medicine Center, Marshfield Clinic Research Foundation, Marshfield, Wisconsin and Department of Biology, University of Central Arkansas Conway, Arkansas
Cynthia Tyler, MS, National Farm Medicine Center Marshfield Clinic Research Foundation, Marshfield, Wisconsin and Department of Biology, University of Central Arkansas Conway, Arkansas
Gwendolyn Anderson, BS, Office of Regulatory Affairs/Arkansas Regional Laboratory, United States Food and Drug Administration, Jefferson, Arkansas
Sarah Phillips, PhD, Office of Regulatory Affairs/Arkansas Regional Laboratory, United States Food and Drug Administration, Jefferson, Arkansas
Khalil Kerdahi, BS, Office of Regulatory Affairs/Arkansas Regional Laboratory, United States Food and Drug Administration, Jefferson, Arkansas
Steven L. Foley, PhD, Office of Regulatory Affairs/Arkansas Regional Laboratory, United States Food and Drug Administration, Jefferson, Arkansas and National Farm Medicine Center, Marshfield Clinic Research Foundation, Marshfield, Wisconsin and Department of Biology, University of Central Arkansas Conway, Arkansas
Reprint Requests: Steven L. Foley, PhD, Marshfield Clinic Research Foundation 1000 North Oak Avenue Marshfield, WI 54449 Tel: 715-389-4012 Fax: 715-389-3808 E-mail: foley.steven{at}mcrf.mfldclin.edu
Objective: Disk diffusion and broth dilution assays are conventionally used for antimicrobial susceptibility testing (AST) of bacteria. The goal of this study was to determine the correlation of results from different AST methods for the Salmonella enterica serovar Heidelberg.
Design: S. enterica serovar Heidelberg (n=105) strains were tested using 4 different AST methods: agar disk diffusion, broth microdilution using Sensititre with the NARMS (CMV1AGNF) panel, manual broth microdilution and Vitek with GNS-207 cards.
Methods: AST was performed using standardized methods and Clinical and Laboratory Standards Institute recommended quality control organisms. Eight drugs were common to all testing methods including amikacin, amoxicillin/clavulanic acid, ampicillin, chloramphenicol, ciprofloxacin, gentamicin, tetracycline and trimethoprim/sulfamethoxazole.
Results: No resistance to amikacin and ciprofloxacin was detected. Overall, the agreement of the AST results among all four methods for the drugs tested was: amikacin (100%), amoxicillin/clavulanic acid (96.1%), ampicillin (97.1%), chloramphenicol (96.2%), ciprofloxacin (100%), gentamicin (80.0%), tetracycline (80.0%) and trimethoprim/sulfamethoxazole (94.3%). There was 97.1%, 95.5% and 98.0% overall agreement between the reference diffusion method and the manual broth microdilution, Sensititre microdilution and Vitek methods, respectively.
Conclusion: The study indicated that AST methods correlated with one another when testing S. enterica serovar Heidelberg isolates, with a few exceptions. In general, discrepancies among the methods were due to isolates being interpreted as intermediately susceptible or due to an increased number of resistances detected with Sensititre and a lower number with Vitek.
Key Words: Antimicrobial susceptibility testing • Broth microdilution • Disk diffusion • Salmonella enterica serotype Heidelberg
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